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	<head>
		<title>Examples</title>
		<script id="bwsl" src="http://ubio.bioinfo.cnio.es/biotools/bws/bwsl.js"></script>
		<?
			include_once('http://ubio.bioinfo.cnio.es/biotools/bws/bwsl.php');
			include_once('../bwel.php');
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	</head>
	<body>
		<h1>Example</h1>
		<h2>Different apoptotic responses of human and bovine pericytes to fluctuating glucose levels and protective role of thiamine.</h2>

		<p>BACKGROUND: Vascular cells in <span id="ihop1"></span> are subjected to daily fluctuations from high to low glucose. We aimed at investigating whether pulsed exposure to different glucose concentrations influences apoptosis in human retinal pericytes (HRP) versus bovine retinal pericytes (BRP), with consequences on the onset of diabetic retinopathy, and the possible protective role of thiamine. METHODS: BRP and HRP (wild-type and immortalized) were grown in physiological/high glucose for 7 days, and then returned to physiological glucose for another 24, 48 or 72 h. Cells were also kept intermittently at 48-h intervals in high/normal glucose for 8 days, with/without thiamine/benfotiamine. Apoptosis was determined through ELISA, TUNEL, Bcl-2, Bax and p53 expression/concentration. RESULTS: Continuous exposure to high glucose increased apoptosis in <span id="ihop2"></span>, but not HRP. BRP <span id="ihop4"></span> normalized within 24 h of physiological glucose re-entry, while HRP apoptosis increased within 24-48 h of re-entry. Intermittent exposure to high glucose increased apoptosis in HRP and <span id="madasmap1"></span>. Bcl-2/Bax results were consistent with DNA fragmentation, while <span id="ihop3"></span> was unchanged. Thiamine and benfotiamine countered intermittent high glucose-induced apoptosis. CONCLUSIONS: Human pericytes are less prone to apoptosis induced by persistently high glucose than bovine cells. However, while BRP recover after returning to physiological levels, HRP protein <span id="sequence1"></span> are more vulnerable to both downwardly fluctuating glucose levels and intermittent exposure. These findings reinforce the hypotheses that (1) glycaemic fluctuations play a role in the development of diabetic retinopathy and (2) species-specific models are needed. Thiamine and benfotiamine prevent human pericyte apoptosis, indicating this vitamin as an inexpensive approach to the prevention and/or treatment of diabetic complications. Copyright (c) 2009 John Wiley & Sons, Ltd.</p>
		
		<h1>Current services available</h1>
		<ul>
			<li><a href="ihop.php">iHOP</a></li>
			<li><a href="fragkb.php">FragKB</a></li>
		</ul>
	</body>
</html>
<script type="text/javascript">
	
	
	$(document).ready(function(){
	
		$.bwe.ihop.instance($('#ihop1'),'diabetes','getRelatedSymbols','iHOP Related Symbols',false);	
		$.bwe.ihop.instance($('#ihop2'),'BRP','getSymbolInfo','iHOP Symbol Info',false);
		$.bwe.ihop.instance($('#ihop3'),'p53','getSymbolInteractions','iHOP Interactions',false);	
		$.bwe.ihop.instance($('#ihop4'),'apoptosis','getRelatedSymbols','iHOP Related Symbols',false);	
		$.bwe.sequence.instance($('#sequence1'),'P48810','getProteinSequence','Sequence',false);	
		
		das_server	= 19;
		pid			= 34;
		dsn			= 'uniprot';
		segment		= 'P08518';  /*Replace the Uniprot ID*/
		size		= 1224;	     /* Replace the protein size */
		
		$.bwe.madasmap.instance($('#madasmap1'),segment,800,300,false);
		
	});	
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